Alcohol based fixitives generally make for good IHC stains requiring no
retriveal.
Patsy Ruegg

"Corazon D. Bucana" wrote:

> Hi All,
>
> I was wondering if anyone has performed immunohistochemistry on cells such
> as buccal mucosa smears fixed with a cytology spray fixative?  If so, what
> antibodies did you use?   I assume the spray fixative contains some type of
> alcohol.  We are planning to do some tests of spray fixed samples because
> the logistics of transporting liquids and specimens is getting more and
> more difficult. Any comments would be greatly appreciated.
>
> Thanks,
>
> Cora Bucana
> Corazon D. Bucana, Ph.D.
> Dept. Cancer Biology
> UT M.D.Anderson Cancer Center
> 1515 Holcombe Blvd, Box 173
> Houston, Texas 77030
> Phone: 713-792-8106
> FAX 713-792-8747