Re: cytology spray fixative and immunohistochemistry

From:Amos Brooks

    I honestly can't comment on the immunocytology of the samples since I am
having a horrible time getting good immuno results with any cytology
specimens. But regarding the spray fixative, I love it. When I was in
cytology using the spray fixative (Addam's Spray Cyte at the time), There
was very little cell loss compared with alcohol immersion. The morphology
was very good too. If the morphology was good I can't imagine the immuno
results to be very adversely affected.
Amos Brooks

----- Original Message -----
From: "Corazon D. Bucana" 
Sent: Thursday, September 27, 2001 3:20 PM
Subject: cytology spray fixative and immunohistochemistry

> Hi All,
> I was wondering if anyone has performed immunohistochemistry on cells such
> as buccal mucosa smears fixed with a cytology spray fixative?  If so, what
> antibodies did you use?   I assume the spray fixative contains some type
> alcohol.  We are planning to do some tests of spray fixed samples because
> the logistics of transporting liquids and specimens is getting more and
> more difficult. Any comments would be greatly appreciated.
> Thanks,
> Cora Bucana
> Corazon D. Bucana, Ph.D.
> Dept. Cancer Biology
> UT M.D.Anderson Cancer Center
> 1515 Holcombe Blvd, Box 173
> Houston, Texas 77030
> Phone: 713-792-8106
> FAX 713-792-8747

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