Steve Machin UK answers
I do formaldehyde induced fluorescence ofor these a long time ago.
The humidity of the formaldehye vapour and very rapid exposure to
this are very important.  Use fresh frozen sections.
If you dont have a chamber which controls humidity then use a
dessicator with dishes of sulphiric acid inside; vary the strength of
acid to vary the humidity.

Best Wishes
Steve Machin UK 



--- Rachel Munshower  wrote: > Hi all you
histnetters,
>   Can anybody help me with getting some info on Catecholamine
> histofluorescence?  We want to be able to see the sympathetic
> innervation
> of the kidney.  Does the histofluorescence diminish severely with
> time?
> Thanks so much!
> Rachel


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