Re: caspase 3

If you are looking for only activated caspase 3 try the R & D catalogue # AF835.
Steam Ag retreival for 25 min, promary Ab incubation of 1 hour, Dako LSAB+ kit
for secondary and SA-HRP for 15 min each.  Dab for 15 minutes.  This will only
detect activated Caspase 3.

Good luck!
Christine Klein
MD Anderson Cancer center

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