Re: TUNEL Expertise Needed
in my experience there all lots of problems with the tunnel assay, in my
experience again, i could never distinquish apoptosis from necrosis and i have
talked to many who have the same problem with this assay.
Ralph Marcucio wrote:
> what kind of "non-specific" staining?? Were all of the nuceli (or an
> excessive number) positive??? I have had the same problem and am trying
> to figure out how to validate the assay.
> Ralph Marcucio, PhD
> UCSF, Ortho. surg.
> On Fri, 28 Sep 2001, Histo-Scientific Research Laboratories wrote:
> > Dear Histonetters,
> > We have a researcher that had sheep myocardial tissue that upon necropsy he
> > put in 70%ETOH. When he went to do his TUNEL staining, he noted nonspecific
> > staining rendering his project useless. Would he be able to 1) cut sections
> > from the processed tissue and post-fix the slides in formalin or
> > paraformaldehyde to make his stain work? 2) If not, could he take the
> > remaining wet tissue that is currently in 70%ETOH and put it in a fixative
> > then process as normal and get results with his TUNEL staining? Your help
> > is greatly appreciated.
> > Sincerely,
> > Tom Galati
> > Histology Laboratory Director
> > HSRL- A GLP Compliant Contract Laboratory
> > 137 S. Main Street
> > Woodstock, VA 22664
> > (540)459-8211
> > fax: (540)459-8217
> > email@example.com
> > www.hsrl.org
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