in my experience there all lots of problems with the tunnel assay,  in my
experience again, i could never distinquish apoptosis from necrosis and i have
talked to many who have the same problem with this assay.
patsy ruegg

Ralph Marcucio wrote:

> what kind of "non-specific" staining??  Were all of the nuceli (or an
> excessive number) positive???  I have had the same problem and am trying
> to figure out how to validate the assay.
>
> Ralph Marcucio, PhD
> UCSF, Ortho. surg.
>
> On Fri, 28 Sep 2001, Histo-Scientific Research Laboratories wrote:
>
> > Dear Histonetters,
> >
> > We have a researcher that had sheep myocardial tissue that upon necropsy he
> > put in 70%ETOH.  When he went to do his TUNEL staining, he noted nonspecific
> > staining rendering his project useless.  Would he be able to 1) cut sections
> > from the processed tissue and post-fix the slides in formalin or
> > paraformaldehyde to make his stain work?  2) If not, could he take the
> > remaining wet tissue that is currently in 70%ETOH and put it in a fixative
> > then process as normal and get results with his TUNEL staining?   Your help
> > is greatly appreciated.
> >
> > Sincerely,
> >
> > Tom Galati
> > Histology Laboratory Director
> > HSRL- A GLP Compliant Contract Laboratory
> > 137 S. Main Street
> > Woodstock, VA  22664
> > (540)459-8211
> > fax: (540)459-8217
> > tomgalati@hsrl.org
> > www.hsrl.org
> >
> >
> >