Re: TUNEL Expertise Needed

From:Ralph Marcucio

what kind of "non-specific" staining??  Were all of the nuceli (or an
excessive number) positive???  I have had the same problem and am trying
to figure out how to validate the assay.

Ralph Marcucio, PhD
UCSF, Ortho. surg.    

On Fri, 28 Sep 2001, Histo-Scientific Research Laboratories wrote:

> Dear Histonetters,
> We have a researcher that had sheep myocardial tissue that upon necropsy he
> put in 70%ETOH.  When he went to do his TUNEL staining, he noted nonspecific
> staining rendering his project useless.  Would he be able to 1) cut sections
> from the processed tissue and post-fix the slides in formalin or
> paraformaldehyde to make his stain work?  2) If not, could he take the
> remaining wet tissue that is currently in 70%ETOH and put it in a fixative
> then process as normal and get results with his TUNEL staining?   Your help
> is greatly appreciated.
> Sincerely,
> Tom Galati
> Histology Laboratory Director
> HSRL- A GLP Compliant Contract Laboratory
> 137 S. Main Street
> Woodstock, VA  22664
> (540)459-8211
> fax: (540)459-8217

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