Re: RCC and Europlakin
Hi Marsha, I have been using RCC from Neomarkers/Labvision for a while now.
It works on my Ventana. I use Protease2 for 8 minutes/32 minute inc with
basic DAB detection. Works great!!! Oh , I ALWAYS use an biotin block with
this Ab. Even RCC can have high amounts of endogenous biotin like activity.
I use the Ab at 1:40. Some companies recommend citrate AR for this ab,
but that doesn't work for me. The enzyme is best in my experience. I can't
remember the clone offhand, I'm home. But if you go to www.labvision.com
you can find it in their online catalog and download the datasheet on PDF.
Labvision also sells a small size vial of virtually all their ab's. This
is great for the bottom line!!! Let's you try it without shelling out big
bucks. Also good for that one of a kind diagnostic or research situation.Good
stuff, good company.
Sorry, I don't have any insight on your Europlakin.
Marsha R Price wrote:
I need any information that you might have on 2 antibodies. RCC and
Europlakin. What clone are you using? Antigen retrieval etc.? Thank you
in advance for your information.
On Fri, 21 Sep 2001 08:37:34 -0500 (CDT) firstname.lastname@example.org
Has anyone done IHC with caspase 3. If so, where was your antibody
what protocol did you use. Thanks
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