Re: RCC and Europlakin

From:

Immunoguy

Hi Marsha, I have been using RCC from Neomarkers/Labvision for a while now. It works on my Ventana. I use Protease2 for 8 minutes/32 minute inc with basic DAB detection. Works great!!! Oh , I ALWAYS use an biotin block with this Ab. Even RCC can have high amounts of endogenous biotin like activity. I use the Ab at 1:40. Some companies recommend citrate AR for this ab, but that doesn't work for me. The enzyme is best in my experience. I can't remember the clone offhand, I'm home. But if you go to www.labvision.com you can find it in their online catalog and download the datasheet on PDF. Labvision also sells a small size vial of virtually all their ab's. This is great for the bottom line!!! Let's you try it without shelling out big bucks. Also good for that one of a kind diagnostic or research situation.Good stuff, good company.

Sorry, I don't have any insight on your Europlakin.

Yours,
Immunoguy

Marsha R Price wrote:

Hi Histonetters,
I need any information that you might have on 2 antibodies. RCC and
Europlakin. What clone are you using? Antigen retrieval etc.? Thank you
in advance for your information.
Marsha Price

On Fri, 21 Sep 2001 08:37:34 -0500 (CDT) r-meyer2@northwestern.edu
writes:

Has anyone done IHC with caspase 3.  If so, where was your antibody 
from and 
what protocol did you use.  Thanks 

Bob Meyer,HTL 
Northwestern University


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