Re: Prostate biopsies/wavy sections

From:Amos Brooks

    You said you use charged slides and Stay-On. I think that is your
problem. Use one or the other. That is probably why the problem is
inconsistent too, Some techs probably use less than others. The reason the
combination of adhesive and charged slides is a problem is that the charged
slides have an ionic charge on them the adhesive is neutrally charged. When
the adhesive coats the slide it nullifies the charge of the slide. You
should use either one or the other.
    My personal preference is to NOT use the adhesive. A proteinaceous
compound may interfere with some immuno stains, and I have noticed that
often if a tech uses too much adhesive, or it wasn't mixed well in the
waterbath, there will be a film of background staining on the slide (not
necessarily on the tissue) which can be distracting and detracts from an
otherwise well prepared slide.
good luck,
Amos Brooks

----- Original Message -----
From: "Cornett, Lorraine D" 
Sent: Wednesday, September 26, 2001 1:25 PM
Subject: Prostate biopsies/wavy sections

> We have been performing 34BE12's on prostate biopsies for over a year.  We
> have just recently started having problems with the sections falling off.
> We do antigen retrieval in EDTA ph 8.0, in the microwave for 2 minutes and
> stand time of 15 minutes.  The problem is somewhat inconsistent which
> it even more confusing.  Our specimens are collected in 10% NBF.   We use
> BioCare Medical charged slides and Surgipath Stay-On in our waterbath's.
> Has anyone experienced this problem, and been able to determine the cause?
> All suggestions will be considered and appreciated!
> Lorraine Cornett HT (ASCP)
> Blue Ridge Pathology Consultants, PC
> 423-224-6767

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