Re: MacCallum - Goodpasture method
Probably got that way heavy breathing on a slide with
aniline oil. I've done the stain and though it is pretty not worth
working with aniline.
Rena Fail
Medical university of Sc
At 12:31 AM 9/25/01 +0100, you wrote:
Steve Machin answers:taining
solution.
As for differentiation, aniline does this very slowly, unlike
acetone
or alcohol. If the section is dry and put into aniline the dye
comes
I don't know why aniline is in the s
out very slowly. But if the section is damp it comes out much
quicker.
We shouldn't do this now because aniline is quite harmful, but the
trick is to do a bit of heavy breathing on the slide. The
moisture
in your breath turns the aniline milky and speeds up the
differentiation. The heavy breathing trick was taught to me by a
drunk Scotsman who will remain nameless.
--- Aidan Schurr <Aidan.Schurr@hvh.co.nz> wrote: > Hi all,
>
> Have an obscure request for a MacCallum - Goodpasture method
for
> Gram positive and negative bacteria. My question: what is
the
> significance of the aniline called for in this method? (both in
the
> staining solution and in the differentiator).
>
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