You could run your slides through avidin/biotin block before staining.
Avidin/Biotin blocks are available through various vendors, I use DAKO
myself. If you bind up the endogenous biotin with the block and then use a
red or blue chromagen, I think you would get your best results.
Glen Dawson BS, HT & IHC (ASCP)
Lead IHC Technologist
From: patsy ruegg [mailto:firstname.lastname@example.org]
Sent: Wednesday, September 26, 2001 9:57 AM
To: sanchez romeo
Subject: Re: hepatocytes
the problem with streptavidin is that what it is labelling is endogenous
biotin and would not be specific for just hepatocytes. it would label any
biotin. biotin can be in places other than hepatocytes.
sanchez romeo wrote:
> Dear Histonetters,
> I am looking for a marker for hepatocytes in pig fetuses for use on frozen
> cryosections (immunoflourescence). I have previously used a commercial
> marker (hepatocyte specific antigen) with minimal success. In my recent
> stainings I noticed that streptavidin-FITC by itself appears to label only
> hepatocytes giving a distinct granular cytoplasmic stain. Is this an
> acceptable marker? Can anyone suggest a reference on endogenous biotin in
> fetal hepatocytes?
> Many thanks in advance.
> Romeo Sanchez (Philippines)
> PhD student
> University of Ghent, Belgium
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