RE: Use of Michel's medium for renal biopsies

From:"Dawson, Glen"

Teresa & All,

I was wondering if Michels can be used safely if it is just after the
expiration date?  I have almost a full bottle that will expire in a month.
It has been in the fridge for the duration and I don't believe that it would
compromise incoming skin or kidney bx.'s  If someone has had problems with
expired Michels, please let me know.

Thanx in advance,

Glen Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
-----Original Message-----
From: Teresa Flores []
Sent: Monday, September 24, 2001 7:05 AM
To: Jarmulowicz, Michael
Subject: Re: Use of Michel's medium for renal biopsies

Michael, yes. In our diagnostic renal pathology em lab, we have used
Michels Transport Media for at least, ten years.
Michels use to be sold under commerical, might still be, Zeus Fixative.
I have the receipe, its no secret as Dr. Michels published it for skin
immunofluorescent biopsies.
There is a drawback of reliability of test after biopsy has been in Michels
Transport Media after five days. But even with that, we have never had a
Let me know,
>I need to provide a renal biopsy service to a remote site.  I am happier
>using immunofluorescence (IF) on fresh frozen tissue rather than
>immunoperoxidase on paraffin processed tissue.  Michel's medium appears to
>be used successfully for skin biopsies and IF, although I have no personal
>experience of its use.
>Can it be used equally well for transport of renal biopsies for IF, and has
>anyone experience of it in this field?
>Michael Jarmulowicz
>Consultant Histopathologist, Royal Free Hospital, London. UK

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