RE: Carnoy's fixative

John Kiernan tells us inter alia:

"The chloroform in Carnoy may not be necessary.
Clarke's fixative, which dates back to the
1850s, is a 3:1 mixture of alcohol and glacial
acetic acid. It has performed as well as or 
better than Carnoy in published comparisons
of fixatives.

All the statements in the preceding paragraphs
relate to appearances in paraffin sections.
For frozen sections or resin embedding, you
need a cross-linking aldehyde fixative such as
formaldehyde, glutaraldehyde or acrolein, but
that's another collection of stories."

Odd that the chloroform is of doubtful utility. Rather like the Bismarck brown in EA stains I guess.

Clarke's sound a bit pricey.

My question after those 2 trivial asides, is what is the effect on immunochemistry?

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire

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