Michel's for TEM-NO!
Michel's is a transport media, not a fixative.
Our laboratory provides Paraformaldehyde-G Fixative for HRLM-TEM and
Michel's Transport Media for immunofluorescent studies.
Nephrologist/surgeon/clinician will divide biopsy and place in appropriate
vials. The most important to our pathologist is the HRLM-TEM, as you can
always do TEM to look for immune deposits. Immunofluorescent is most
important for IgA or an IgG nephropathy.
We have had to process biopsy submitted in Michel's for HRLM-TEM and the
morphology is terrible! Worse than 10% Formalin.
Many thanks Teresa and all,
I note you say in your EM lab. Do you recover the biopsy from Michels to
then process to em as well as the immunofluorescence? I am trying to decide
the best way to advise the remote site. Locally we immediately divide the
specimen for routine paraffin, EM and IF. If the remote site can just send
the biopsy in a single medium and we divide it here then that would solve a
lot of potential problems.
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