I've been having a terrible time with endogenous biotin in spleen.
Yesterday i tried Phyllis Davis' biotin blocking recipie (posted 5/01).
The Grocery store concoction of eggs and milk is so wonderfully simple.
It did fail however to knock out all non-specific label in my NBF fixed
paraffin embedded mouse spleen.
The non-specific label i'm getting localizes in cells that look a bit like
macrophages. I'm used to looking at skin-not spleen. Does anyone have any
suggestions?
Thanks!
Lara
Dermatology Dept
University of Washington Seattle, WA
muffley@u.washington.edu

On Fri, 7 Sep 2001 DDittus787@aol.com wrote:

> Almost all tissue has biotin, however :liver , and spleen, seem to be the
> worst.
>                                                 Dana
>