Sequential HRP staining

From:Gayle Callis

It is possible.  You can do a second peroxidase block AFTER development of
first chromogen to quench any residual peroxidase from HRP step.  Develop
first chromogen color, go back to PBS rinse, then do a second endog
peroxidase block, and proceed with second HRP protocol.  

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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