Re: vibratome sections
Have you tried changing the speed or the frequency of the knife? A good
consistency for tissue is similar to the white in a hard boiled egg! What
is the consistency of the lung, harder or softer than the egg white? Is the
inside a different consistency than the outside?
Leica Specimen Preparation
----- Original Message -----
From: "Judy Trogadis"
Sent: Thursday, August 30, 2001 6:30 AM
Subject: vibratome sections
I am trying to cut sections of rat lung tissue, about 200 microns thick,
with a vibratome. It is difficult, to say the least, because the lung was
inflated just prior to fixation and, although well fixed, retained a spongy
consistency and therefore not firm enough to section. I have embedded it in
agar without any improvement.
Does anyone have any suggestions.
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8
ph: 416-864-6060 x6337
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