Re: speaking of spleen


sometimes you have to go to a non-AB labeling system.  i like envision from
dako, it does not use AB but a labeled polymer and therefore gets around the
end. biotin problem.
patsy ruegg

"L. Muffley" wrote:

> I've been having a terrible time with endogenous biotin in spleen.
> Yesterday i tried Phyllis Davis' biotin blocking recipie (posted 5/01).
> The Grocery store concoction of eggs and milk is so wonderfully simple.
> It did fail however to knock out all non-specific label in my NBF fixed
> paraffin embedded mouse spleen.
> The non-specific label i'm getting localizes in cells that look a bit like
> macrophages. I'm used to looking at skin-not spleen. Does anyone have any
> suggestions?
> Thanks!
> Lara
> Dermatology Dept
> University of Washington Seattle, WA
> On Fri, 7 Sep 2001 wrote:
> > Almost all tissue has biotin, however :liver , and spleen, seem to be the
> > worst.
> >                                                 Dana
> >

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