Re: another positive control
Another thing to consider on those macrophages is that the coloration
may not be any sort of non-specific "staining" at all. Macrophages eat a
lot of pigmented stuff (hemosiderin, melanin, anthracotic pigment, etc.)
which naturally remains pigmented throughout the immuno procedure. Check
your H&E; if the pigment is present there, it CANNOT be an artifact of the
immunoperoxidase procedure.
As far as a positive control for macrophages; besides spleen, lung can
be a good control, but there's no guarantee that the alveolar macrophages
won't be pigment-laden as well. I am not specifically familiar with MOMA-2,
however.
Best of luck,
Phyllis Davie
PhenoPath Laboratories--Seattle, WA
pdavie@phenopath.com
---------------
on 9/7/01 3:30 PM, L. Muffley at muffley@u.washington.edu wrote:
> Thanks to everyone for the advice on the non-specific label in mouse
> spleen!
>
> Also- does anyone have a recommendation for positive control tissue for a
> macrophage marker (specifically MOMA-2).
> Lymph node? (can i get around using spleen?)
>
> Eventually i want to use MOMA-2 on NBF fixed mouse tissue (wounds).
>
>
>
> Lara Muffley
> Dermatology Dept
> University of Washington
> Seattle, WA
> muffley@u.washington.edu
>
>
>
>
>
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