Re: Sequential HRP staining

From:Karen Pawlowski

Hi Gayle,

I didn't see the first posting of this, but I was just wondering about
this the other day.  Can you do a double staining if you are using a 
mouse-on-mouse kit?  Both antibodies are made in mice and so the 
secondary antibody is the same for both.

Karen Pawlowski, Ph.D.

Gayle Callis wrote:
> It is possible.  You can do a second peroxidase block AFTER development of
> first chromogen to quench any residual peroxidase from HRP step.  Develop
> first chromogen color, go back to PBS rinse, then do a second endog
> peroxidase block, and proceed with second HRP protocol.
> Gayle Callis
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)

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