Re: Mulligan's stain
You say even in a fume hood you get vapors from this procedure??? On my
fume hood, the baffle has a setting for different vapors --- vapors lighter
than air and those heavier than air. Check your fume hood to see if you
can adjust the baffle. If so, I would try the heavier than air setting for
CuSO4. I use my fume hood for all sorts of stinky things and I never get
so much as a whiff of vapors coming from the hood.
Just a thought :-)
Connie McManus
At 12:54 PM 9/5/01 -0230, J. Foote wrote:
>
>Hi,
>
>I have been asked to prepare and stain the grey matter of 4 mm slices of
>brain for macroscopic study in a neuroscience course that will be taught
>this semester. The method I have is a variation on a Mulligan stain that
>was published by Roberts and Hanaway in Stain Technology 44(3). This
>method has been used for the last thirty years with good results, but the
>down side of the procedure is the production of strong vapors. The method
>requires CuSO4-phenol mixture followed by quick wash in potassium
>ferrocyanide. I have been told even with full safety measures taken (i.e..
>use of gowns, respirators, double layer of gloves and the use of fume
>hoods) it is still an unpleasant procedure. So, I was wondering does anyone
>on this list have any experience staining whole brain slices and if they
>do, can they suggest other methods that might not be as unpleasant. I look
>forward to any suggestions you might have.
>
>J. Foote
>
>J. Foote
>Histology Unit
>Medical School Laboratories
>Faculty of Medicine
>Memorial University of Newfoundland
>St. John's, Newfoundland
>
>
>
>
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA
(435) 797-1891
fax (435) 797-2805
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