What antibodies does he want to use?. I have done
immunos on Bouins fixed tissue sections. My experience
is that it really depends on the antibody in question.

James Mubiru
UTHSCSA


--- "Corazon D. Bucana" 
wrote:
> Hi All,
> 
> A colleague needs to perform immunohistochemistry on
> some precious samples
> which unfortunately were previously fixed in Bouin's
> fixative.  I would
> appreciate any comments or suggestions on antigen
> retrieval if it is
> applicable.  
> 
> Thank you,
> 
> Cora Bucana 
> Corazon D. Bucana, Ph.D.
> Dept. Cancer Biology
> UT M.D.Anderson Cancer Center
> 1515 Holcombe Blvd, Box 173
> Houston, Texas 77030
> Phone: 713-792-8106
> FAX 713-792-8747
> 


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