RE: apoptosis reagent/kit

From:"Baldridge, Lee Ann"

Hi Deb,
I too use the Roche kit. I've found that I usually have to dilute the TUNEL
reaction mix 1:3. Make sure that your temp. is correct for the incubation
and don't let the slides dry out.  I use plastic coverslips that came with
another companies Apop. kit  Hope this is of some help.

Lee Ann Baldridge
IU School of Medicine 

-----Original Message-----
From: []
Sent: Thursday, September 06, 2001 4:13 AM
To: Debra Dunlap
Subject: Re: apoptosis reagent/kit

Hi Deb,

 I use, for the apoptosis, the" In Situ Cell Death Detection POD Kit" made
from Roche Molecular Biochemicals  Cat No. 1 684 817

Best regards


Stefano Mantero
Molecular Morphogenesis Laboratory
National Institute for Cancer Research
Largo Rosanna Benzi, 10
16132  Genova
Ph.  +39-010-5737-234
Fax; +39-010-5737-224

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