Hi Deb,
I too use the Roche kit. I've found that I usually have to dilute the TUNEL
reaction mix 1:3. Make sure that your temp. is correct for the incubation
and don't let the slides dry out.  I use plastic coverslips that came with
another companies Apop. kit  Hope this is of some help.

Lee Ann Baldridge
IU School of Medicine 
IHC Lab
Indpls.IN.
317-274-5790

-----Original Message-----
From: mantero@vega.cba.unige.it [mailto:mantero@vega.cba.unige.it]
Sent: Thursday, September 06, 2001 4:13 AM
To: Debra Dunlap
Cc: histonet@pathology.swmed.edu
Subject: Re: apoptosis reagent/kit


Hi Deb,

 I use, for the apoptosis, the" In Situ Cell Death Detection POD Kit" made
from Roche Molecular Biochemicals  Cat No. 1 684 817

Best regards

Stefano

Stefano Mantero
Molecular Morphogenesis Laboratory
National Institute for Cancer Research
Largo Rosanna Benzi, 10
16132  Genova
ITALY
Ph.  +39-010-5737-234
Fax; +39-010-5737-224