RE: Endogenous Biotin and Avidin
Liver and kidney are most likely to contain endogenous biotin. Avidin is
from hens eggs and is not a problem. You can block endogenous biotin with
unlabeled avidin. See Woods GS, Warnke R. (1981) Suppression of endogenous
avidin-binding activity in tissues and its relevance to biotin-avidin
detection systems. J Histochem Cytochem 29:1196.
I also vaguely remember an article suggesting that mast cells bind to the AB
complex as well but can't trace it.
St Michael's Hospital
From: Steve Machin UK [SMTP:email@example.com]
Sent: 07 September 2001 12:29
To: Histonet Histonet Histonet
Subject: Endogenous Biotin and Avidin
Could someone please give me a list of tissues which are likely to
give background in ABC Kits because they contain endogenous Biotin
Steve Machin UK
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