Cindy,
	I work on various animal systems with various in house and
commercial antibodies. I have not done a controlled study, but I have done
enough with different antibodies to know that the strength of the staining
can vary with precut specimens. In my spare time I would like to do precuts
and hold some and RT and some at -80 then do staining at increasing time
points. Now I just cut my control sections when  I run the IHC. 

Cynthia Favara



-----Original Message-----
From: Cindy Chard-Bergstrom [mailto:ChardB@vet.ksu.edu]
Sent: Monday, August 27, 2001 9:19 AM
To: histonet@pathology.swmed.edu
Subject: BVD staining


I am experiencing problems with our BVD (bovine viral diarrhea) stain. My
controls are precut, sometimes a week in advance. Slides are not heated
until right before they are stained. At first the control stains very nice,
but over time the staining on subsequent runs is diminished. Only a few
cells will stain. I make up the antibody dilution fresh each time I use it.
Is anyone else doing this stain and if so have  you experienced this
problem? Any suggestions, anyone?



Cindy Chard-Bergstrom B.S.,HT(ASCP),QIHC
Microbiologist III 
Kansas State University
chardb@vet.ksu.edu