Cyclin D1

From:"C Sawrenko"

Hello Histonetters,
I'm in need of more your valuable input.  For those using Cyclin D1, do your
sections show a cytoplasmic blush as well as nuclear staining?  If so, is
this an undesirable result or something that is acceptable?  We are using
tonsil as a control as well as staining tiny bronchial biopsies.  The
epithelium consistently shows some cytoplasmic blush as well as nuclear
staining.  I have tried a protein block which helps somewhat but not
entirely. We use Envision+ for detection.
Thanks in advance for you help.

Chris Sawrenko
Histopathology, BC Cancer Agency
Vancouver, BC, Canada

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