I am experiencing problems with our BVD (bovine viral diarrhea) stain. My controls are precut, sometimes a week in advance. Slides are not heated until right before they are stained. At first the control stains very nice, but over time the staining on subsequent runs is diminished. Only a few cells will stain. I make up the antibody dilution fresh each time I use it. Is anyone else doing this stain and if so have  you experienced this problem? Any suggestions, anyone?



Cindy Chard-Bergstrom B.S.,HT(ASCP),QIHC
Microbiologist III 
Kansas State University
chardb@vet.ksu.edu