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| From: | Josefa Nava <pepanava@hotmail.com> |
From: HistoNet Server <histonet@pathology.swmed.edu>
To: HistoNet Server <histonet@pathology.swmed.edu>
Subject: Daily Digest
Date: Tue, 19 Sep 2000 00:09:52 -0500 (CDT)
----------------------------------------------------------------------
Date: 18 Sep 2000 04:01:55 -0500
From: "Richard Pitman" <Richard.Pitman@wri-tr.wmids.nhs.uk>
Subject: Re: rhodamine
I hope BDH don't object to me quoting from their data sheet:
BDH HAZARD DATA SHEETS PAGE - 1
+------------------------------------------------------------------------+
Product : Rhodamine B
+------------------------------------------------------------------------+
CAS Number : 81-88-9
+-----------------------------------------------------------------------+
Product Codes : 13111-34142- - - - - - -
- - - - - - - -
+-----------------------------------------------------------------------+
HAZARD IDENTIFICATION
Symbols : Xn Phrases : R20/22
S24/25
TRANSPORT INFORMATION
Hazard Class : NR UN Number : Pkg Group :
COMPOSITION : Organic dye
REGULATORY INFORMATION : Not implemented
PHYSICAL AND CHEMICAL PROPERTIES
Description : Green crystals or dark reddish violet powder
Melting Pt : 165 #161#CBoiling Pt : n/aSpecific Gravity : n/a
Solubility in Water : Soluble
Vapour Pressure : n/a
Vapour Density : n/a
FIRE AND EXPLOSION HAZARD May evolve toxic fumes in fire
Flash Point : n/a
Explosive Limits : n/a
Auto-Ignition Temperature : n/a
FIREFIGHTING MEASURES Not applicable
HEALTH HAZARDS Harmful by ingestion and inhalation. Stains. May irritate
eyes
and skin.
TOXICOLOGICAL INFORMATION
Toxicity Data : LD50 887 mg/kg oral, mouse
Carcinogenicity : Has been found to cause cancer in laboratory animals
Mutagenicity/Teratogenicity :
Evidence of mutagenic effects
>>> Tibor Ric <tibor@home.se> 09/16/00 06:27am >>>
Hi!
Does anybody knows if rhodamine dye is carcinogen? I've heard that it is. Is
it true?
If You do this staining, please could I get Your recipe?
And one more thing. Where could I find a good histological resource on the
Internet?
Thanks in advance!
Tibor Ric
Medical Laboratory Technologist (BMA)
Sweden, Karlstad
----------------------------------------------------------------------
Date: 18 Sep 2000 09:11:50 -0500
From: Nick_Madary@hgsi.com
Subject: Re: histology educational texts
Are you the Jerry Duncan that used to work at the AFIP 20 yrs ago? I
believe you were Navy. Anyway, are you talking about the old brown Preece?
The ISBN is 0-316-71765-7.
----------------------------------------------------------------------
Date: 18 Sep 2000 09:42:28 -0500
From: "Su, Phy-Huynh" <psu@shctampa.usf.edu>
Subject: Job Opportunity in Shriners Hospital for Children in Tampa, Fl
Center for Research in Skeletal Development and Pediatric Orthopaedics at
the Shriners Hospital in Tampa is in need of an experienced Bone
Biologist/Histologist with experience in working with plastic embedded bone
sections for histomorphometry. The candidate will work as a research
associate or scientist (depending on his/her experience and ability to work
independently) on a funded research project. The position involves working
wih an experimental model of ischemic necrosis of femoral head to
quantitatively assess bone resorption and formation. The salary ranges from
$25,000 to $35,000, depending on the experience and qualifications.
Benefits (medical, dental, life insurance, paid time off, etc) are included.
The position is available now. If you are interested please send resume/CV
to:
Human Resources
Shriners Hospital for Children
12502 North Pine Drive
Tampa, Fl 33612
Fax: 813-975-7129
e-mail: hkim@shrinenet.org
----------------------------------------------------------------------
Date: 18 Sep 2000 10:53:22 -0500
From: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
Subject: RE: decloaker & bizarre name
They're Trekkies! ... no kidding.
Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI 53792-2472
(608)265-6596
FAX: (608)263-1568
> -----Original Message-----
> From: Laurie Brett [SMTP:lauriebrett@bigfoot.com]
> Sent: Saturday, September 16, 2000 1:05 PM
> To: histonet histonet@pathology.swmed.edu>
> Subject: decloaker
>
> Thanks to all those who enlightened me - bizarre name though!
>
> --
> lauriebrett@bigfoot.com
>
----------------------------------------------------------------------
Date: 18 Sep 2000 11:35:51 -0500
From: "Terrett, Barb" <Barb.Terrett@uhn.on.ca>
Subject: galectin-3
Hi all,
Is anyone out there using a commercially available galectin-3? Any help
would be appreciated.
Thanks b
----------------------------------------------------------------------
Date: 18 Sep 2000 12:04:21 -0500
From: Patsy Last <PLast@pathgroup.com>
Subject: FW: subscribe
> -----Original Message-----
> From: Patsy Last
> Sent: Monday, September 18, 2000 11:32 AM
> To: Patsy Last; 'histonet@pathology.swmed.edu'
> Subject: RE: subscribe
>
> We have an immediate need to fill a couple of Histologist positions at
one
> of our Nashville, TN hospital laboratory locations. These positions
offer
> full-time employment with a need to work alternating weeks - week one:
> Monday -
> Friday from 10:00 PM to 6:00 AM; week two: Sunday - Thursday from 10:00
PM
> to 6:00 AM. We require a minimum of one (1) year Histology experience;
> certification not required. We offer competitive salaries and an
> excellent benefits package including vacation and sick leave, medical,
> dental, vision, LTD, company-paid life insurance and 401(k).
>
> Qualified and interested candidates should mail or fax resume and salary
> requirements to:
>
> PathGroup, Nashville, Inc.
> ATT: H.R. Director
> SouthPointe Bldg.,
> 5301 Virginia Way, Suite 300
> Brentwood, TN 37027
> Fax: (615) 221-4449
> e-mail: plast@pathgroup.com
>
>
> -----Original Message-----
> From: Patsy Last
> Sent: Monday, September 18, 2000 11:17 AM
> To: 'histonet@pathology.swmed.edu'
> Subject: subscribe
>
>
----------------------------------------------------------------------
Date: 18 Sep 2000 13:35:34 -0500
From: "marjorie lehman" <Marjorie.Lehman@unilever.com>
Subject: RE: decloaker & bizarre name
Don't you love it?? I just received their "What's New"" flyer with the new
products spotlight on "BORGdecloaker .... even the most resistant antigens
will
be assimilated. Truly, resistance is futile."
- -----Original Message-----
From: Sebree Linda A. [SMTP:la.sebree@hosp.wisc.edu]
Sent: Monday, September 18, 2000 11:30 AM
To: 'Laurie Brett'
Cc: 'Histonet'
Subject: RE: decloaker & bizarre name
They're Trekkies! ... no kidding.
Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI 53792-2472
(608)265-6596
FAX: (608)263-1568
> -----Original Message-----
> From: Laurie Brett [SMTP:lauriebrett@bigfoot.com]
> Sent: Saturday, September 16, 2000 1:05 PM
> To: histonet histonet@pathology.swmed.edu>
> Subject: decloaker
>
> Thanks to all those who enlightened me - bizarre name though!
>
> --
> lauriebrett@bigfoot.com
>
----------------------------------------------------------------------
Date: 18 Sep 2000 13:35:49 -0500
From: "Macke, Gail" <gmacke@shrinenet.org>
Subject: RE: Job Opportunity in Shriners Hospital for Children in Tampa, F l
Histonetters,
In regards to the job opportunity at Shriners Hospital in Tampa, I
would
not have any misgivings about working for the Shriners.
I currently work for Shriners Hospital in Cincinnati, Ohio. They are
great people to have as your employer. I don't know what the Tampa unit is
like, but working here in Cincinnati has been great. I do the histology
for the whole research floor and work with several different researchers.
Yes, at times it gets a little down, but then our patients are all burn
victims (just four of the units are burn, the other 19 are orthopedic
units). The organization as a whole is a great place. There are many
benefits to working for the Shriners Hospitals for Children, both personal
and work wise. Our health and dental benefits are very good; vacation/sick
time is also just as good as the medical coverage. Please, if you are
thinking about it, go for it!! There are so many kids who need your help
and knowledge, and when you see them smile and laugh because of something
you've done-- no matter how small or big it is-- you'll be glad you took the
job.
Sincerely,
Gail Macke,HTL ---Cincinnati Unit
> -----Original Message-----
> From: Su, Phy-Huynh [SMTP:psu@shctampa.usf.edu]
> Sent: Monday, September 18, 2000 10:13 AM
> To: 'Histonet'
> Subject: Job Opportunity in Shriners Hospital for Children in Tampa,
> Fl
>
> Center for Research in Skeletal Development and Pediatric Orthopaedics at
> the Shriners Hospital in Tampa is in need of an experienced Bone
> Biologist/Histologist with experience in working with plastic embedded
> bone
> sections for histomorphometry. The candidate will work as a research
> associate or scientist (depending on his/her experience and ability to
> work
> independently) on a funded research project. The position involves
> working
> wih an experimental model of ischemic necrosis of femoral head to
> quantitatively assess bone resorption and formation. The salary ranges
> from
> $25,000 to $35,000, depending on the experience and qualifications.
> Benefits (medical, dental, life insurance, paid time off, etc) are
> included.
> The position is available now. If you are interested please send
> resume/CV
> to:
>
> Human Resources
> Shriners Hospital for Children
> 12502 North Pine Drive
> Tampa, Fl 33612
> Fax: 813-975-7129
> e-mail: hkim@shrinenet.org
>
----------------------------------------------------------------------
Date: 18 Sep 2000 15:15:48 -0500
From: Heike Grabsch <h.grabsch@uni-koeln.de>
Subject: [Fwd: blueing]
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Date: Mon, 18 Sep 2000 17:34:04 +1100
Subject: blueing
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Hi,
I have just discoveedr the histonet group, but can't find any subscription
or mail-to details, so I thought I would mail you directly.
The branching artefact you describe, you also suggest does not wash out in
water/buffer etc. Not quite what one might expect if this was
chemical/crystalline in nature.
Some years ago I had very nice branching haematoxylin positive artefact all
over all my slides. I eventually realised it was fungi, that had been
imprinted on the el cheapo Chinese (standard routine issue) slides from the
interleaving rice paper. Still there despite normally appropriate cleaning.
It does seem plausible, that there may be a growth occuring from the
prolonged (and by the sound of it rather grotty) tap water washing, hot at
that. Whether the contaminant arose from the water, or potentially from the
LiCO3 stock (fungi love alkali yes?), the tap water sounds like nutrient
soup.
A fresh batch of LICO3 would be an easy and quick check. Have also seen
TRIS buffer stock for immuno contaminated with yeast.
No telling how long the LiCO3 has been around by the sound of the absent
normal lab person.
Just a possible explanation
regards
Chris Brown
Sydney
ps if this sounds relevant, or even of some amusement value, perhaps you
could forward it to the group
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----------------------------------------------------------------------
Date: 18 Sep 2000 17:56:22 -0500
From: rcarpenter@mercury.ih.org
Subject: schedule for TP1050
Greetings Histoneters,
I now have in my lab a demo Lieca TP1050 tissue processor. This is
the first time in 20 years of histopathology that I have ever used an
automated machine. I have to say I really like it, but now I have to
figure the thing out. The first question I have is, does anyone have a
good schedule for small mouse tissue. We run the full course of tissue and
we put multiple pieces in a cassette. With my old TP1010 the tissues were
always somewhat overprocessed, especially the livers and brain. What sort
of schedules do folks with more experience use for these types of tissue?
Temperature? Vacuum/Pressure?
Right now I am using HemoDe (Citrosolve) for the clearing. The
only reason I am using it is becuase my TP1010 is not vented and people in
my lab would not care for the xylene smell. With the new machine I plan on
switching back to xylene for a couple of reason. One reason is to extend
the life of my paraffin. The other is that I don't think HemoDe performs
very well as a clearing agent period. I seems to load up with fat very
quickly. It also stays in the paraffin because of it's low vapor pressure.
Nasty. The question is am I missing something about HemoDe?
Any help you folks can give me would be appreciated.
Randy Carpenter
Parker Hughes Institute
Roseville, MN
----------------------------------------------------------------------
Date: 18 Sep 2000 19:17:05 -0500
From: VICKI.GARCIA@ROCHE.COM
Subject: H&E stain for hard plastic
Hi all-
I am sectioning bone (rat proximal tibia) embedded in methylmethacrylate and
I need to stain with H&E. I do not have an H&E protocol for hard plastic
sections. Could someone help me out?
Thanks so much,
Vicki Garcia
Roche Bioscience
Musculoskeletal Research
(650) 855-5972
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