re:Subject: In-situ hybridization (ISH) on paraffin sections

From:"Brothers, Rachel" <rachel_stock@internet.organo.com>

     Date: 19 Sep 2000 15:00:47 -0500
     From: Miriam Schroeder <vitalred@yahoo.com>
     Subject: In-situ hybridization (ISH) on paraffin sections
     
     >Hi.  I was wondering if anyone has experience with
     >in-situ detection of mRNA in paraffin-embedded tissue.
     > In particular, I wondering about the necessity of
     >RNase-free methods in the tissue PREPARTION
     >(processing, cutting, etc.) ..........
     
     If anyone is responding to Miriam's question above, please post to the 
     Listserver too. I am trying ISH (DNA) for the first time and could use 
     all the tips you experts can share!
     -Rachel Stock




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