processing mouse tissues

From:Michelle Peiffer <mlk101@psu.edu>

Hi all,

Does anyone have a foolproof processing schedule for mouse tissue, which
they could share with me?  We are a research lab embedding mouse liver,
kidney, pancreas and brain for IHC.  

I want to avoid using xylene, it gives me a wicked headache, and our
ventilation is not adequate for all these automatic processing machines to
be filled with xylene.  I am currently using histosolve, but I would be
interested to know what substitutes others are using and if there is any
difference.

Thanks in advance.

Michelle Peiffer
*************************************************************
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email:  mlk101@psu.edu
**************************************************************
 



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