mast cell stain

From:Mike Bromley <>

I have a reference for the use of aminocaproate esterase:
Am J Clin Pathol 73:48-54 (1980)
'Eosinophilia in systemic mastocytosis' by LT Yam, CF Yam and CY Li
I have used the stain to identify mast cells in glycol methacrylate embedded
tissue (see Arthritis and Rheumatism 27: 857-863 (1984) 'Histopathology of
the Rheumatoid Lesion'  M Bromley and DE Woolley

I don't think the stain works well on routine formalin fixed paraffin
embedded tissue. Also I don't think the substrate is commercially available.

Best Wishes

Mike Bromley PhD
Histology Department
Paterson Institute
Wilmslow Road
M20 9BX
Original message:

Date: 20 Sep 2000 09:36:47 -0500
From: "Nader, Alexander" <
<> >
Subject: Mast cells

recently I heard from a colleague about a method for mast cell staining in
human. He told me about aminocaproate-esterase staining, which should be
very specific.
I cannot find anything about this stain neither in AFIP's, Bancroft,
Kiernan, Burckhardt or Romeis.
Can someone send me a recipe of this stain.
Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
Heinrich-Collin Strabe 30
A-1140 Wien, Osterreich
+43-1-910 21 DW:2422

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