We have also had a problem with Eosin bleeding on routine H&E slides.  We
use Xylene on the stainer and Mounting Medium from Richard-Allan on an
automated coverslipper.  We have three absolute alcohol stations after the
Eosin and have tried using fresh alcohols and xylenes for each staining
rack, but still have the problem.  Sometimes it is more noticeable than
others.  Could it be the mounting media?  I would appreciate any
suggestions.

Thanks,
Teresa

 
Teresa P. Wylie
Scientist
Medicine Safety Evaluation
GlaxoWellcome
RTP, NC