|From:||Gayle Callis <firstname.lastname@example.org>|
I am very curious about WHY the quality of trichrome is better with xylene
rather than Propar. I found no difference between this xylene substitute,
Clearite 3 or xylene.
I do extend timing in substitutes because they tend to be more water
sensitive and rotate them more frequently, but the stain was no different.
Date: Mon, 25 Sep 2000 11:33:34 -0400
>From: "Hess, Mary" <email@example.com>
>Subject: Xylene Substitutes
>To: "'firstname.lastname@example.org'" <email@example.com>
>Hello, this is in response to the recent xylene substitute questions. We
>currently use Pro-Par from Anatech for all deparaffining and clearing steps
>for every stain except Masson's Trichrome, where we use xylene instead. We
>just purchased an automatic slide stainer and are trying to put our three
>major stains on the machine at once, which means we need to combine the
>deparaffining and clearing steps. I like xylene for the quality of our
>trichrome, but man, its nasty stuff. Is there a way to use pro-par (or any
>xylene substitute really) for deparaffining and xylene for the last
>clearing stage? I will be running test slides trying to figure this out, but
>I'm hoping someone might have already have tried combining xylene and other
>xylene substitutes. My gut feeling is use one or the other but I might get
>lucky. By the way, thanks for the help with the H&E slide stainer
>information a couple of months back, we purchased the Mikrom Robot stainer.
>-Mary Hess, San Diego, Ca.
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
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