Xylene Substitutes

From:Gayle Callis <uvsgc@msu.oscs.montana.edu>

I am very curious about WHY the quality of trichrome is better with xylene
rather than Propar.  I found no difference between this xylene substitute,
Clearite 3 or xylene.

I do extend timing in substitutes because they tend to be more water
sensitive and rotate them more frequently, but the stain was no different.  

Date: Mon, 25 Sep 2000 11:33:34 -0400
>From: "Hess, Mary" <mary.hess@canji.com>
>Subject: Xylene Substitutes
>To: "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
>Hello, this is in response to the recent xylene substitute questions. We
>currently use Pro-Par from Anatech for all deparaffining and clearing steps
>for every stain except Masson's Trichrome, where we use xylene instead. We
>just purchased an automatic slide stainer and are trying to put our three
>major stains on the machine at once, which means we need to combine the
>deparaffining and clearing steps. I like xylene for the quality of our
>trichrome, but man, its nasty stuff.  Is there a way to use pro-par (or any
>xylene substitute really)  for deparaffining and xylene for the last
>clearing stage? I will be running test slides trying to figure this out, but
>I'm hoping someone might have already have tried combining xylene and other
>xylene substitutes. My gut feeling is use one or the other but I might get
>lucky. By the way, thanks for the help with the H&E slide stainer
>information a couple of months back, we purchased the Mikrom Robot stainer.
>-Mary Hess, San Diego, Ca. 
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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