From:Joan Yonchek <>

I am interested in hearing from anyone versed in the use of a Vibratome.
I have been asked to obtain 10-20 micron thick sections of small pieces (1mm
cubes) of formalin fixed skin. Specifically I would like to know the speed,
amplitude and blade angle you would recommend as well as the method for
embedding the tissue in agarose.  Also, do you use a sapphire knife, a
single edge injector type razor blade or a double edge type razor blade?  Is
it necessary to remove the agarose from the section before staining?  What
type of
slides are best?  Do you prefer staining the free-floating section and then
adhering it to the slide or dry the section on the slide before staining?

Other than that I got it covered!


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