We also occasionally experience this problem and would be interested in the 
responses.



At 01:46 PM 9/26/2000 -0400, Wylie, Teresa P wrote:
>We have also had a problem with Eosin bleeding on routine H&E slides.  We
>use Xylene on the stainer and Mounting Medium from Richard-Allan on an
>automated coverslipper.  We have three absolute alcohol stations after the
>Eosin and have tried using fresh alcohols and xylenes for each staining
>rack, but still have the problem.  Sometimes it is more noticeable than
>others.  Could it be the mounting media?  I would appreciate any
>suggestions.
>
>Thanks,
>Teresa
>
>
>Teresa P. Wylie
>Scientist
>Medicine Safety Evaluation
>GlaxoWellcome
>RTP, NC
>
>
>


**************************************************************************
Jill Songer HT (ASCP)
Anatomic Pathology Lab Supervisor
Veterinary Teaching Hospital
Virginia Tech
Blacksburg, Virginia 24061