Hi Cyrla-

I don't have any great advice on how to get a better Cresyl on
the emulsion dipped slides; but, I do know the emulsion will
slough off in the Cresyl and give the Cresyl stain a short
life. When I have had to stain emulsion-dipped slides I kept a
separate Cresyl for that purpose. I would not use the same Cresyl
to stain other slides.

Good luck-

Lorraine Gibbs
Physiology & Biophysics
UW

On Wed, 27 Sep 2000 Cyrla.Hoffert@astrazeneca.com wrote:

> Hi, all
>  
> Does anyone have experience with cresyl violet acetate staining of post- in
> situ hybridization (on fresh frozen sections) slides that have been dipped
> in Kodak emulsion?  A long time back, 2 strange things started to happen.
> First, I would sometimes get pink spots here and there on random slides
> within and/or between racks.  This is a pretty rare event.  Second, I
> consistently get this opacity or haze appearing mainly over the tissue.
> This is often accompanied by spaces or holes in the emulsion.  This is not
> happening on the back side of the slide.  Has anyone else has come across
> these phenomena?  I have already tried changing solutions, stocks, lots and
> times.  I use xylene substitute and have tried 2 different brands already.
> I have considered all light sensitivity aspects.  I have stopped diluting
> the emulsion with water and more...
> 
> I would really appreciate any suggestions since this haze is essentially
> ruining much of my work.  Thanks in advance!
> 
> C
> 
> 
> Cyrla Hoffert, MSc.
> AstraZeneca R&D Montreal
> 7171 Frederick Banting
> Ville St. Laurent (Montreal), Quebec
> H3S 1Z9
> 
> * 514.832.3200 ext. 2122 
> FAX 514.832.3232
> 
> 
>