I always keep a camel hair brush by my waterbath and when I see bubbles forming on the bottom I use the brush to loosen the bubbles and then wipe the surface of the waterbath with a kimwipe.  I have seen more bubbles form with distilled water than tap water.  They are worse after microwaving the distilled water or after the DI tanks are changed.    Sarah

Sarah Christo, HT (ASCP)
Research Associate, Histology Lab
Texas A&M University
College of Veterinary Medicine
Dept. of Vet Anatomy & Public Health
College Station, TX  77843-4458
phone: (979) 845-3177
fax:  (979) 458-3499

>>> Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt> 09/22/00 03:46AM >>>
Histonetters

I have been working in histopathology for about sixteen years and now a very 
annoying problem has cropped up. We are getting bubbles floating up to the 
sections in the floatation baths. They are not big bubbles that are usually seen 
after we change the distilled water. For those we simply tap on the bath and 
they are easily removed. These are very tiny and cover the underside of the 
whole section.
Any ideas of how they can be prevented from forming or removed?
Thanks for your help

Carmen
Malta