Re: Xylene substitutes

From:Sarah Christo <>

Dear Mary,
   I'm not familiar with Refrax mounting media, but if it is a clear media, it is not compatable with Pro-Par.  You have to use one of the yellow medias like Coverbond or Permount.  The mounting media is making the stains bleed.  I have been doing Trichromes for years with Pro-Par without any problems.  Sarah.

Sarah Christo, HT (ASCP)
Research Associate, Histology Lab
Texas A&M University
College of Veterinary Medicine
Dept. of Vet Anatomy & Public Health
College Station, TX  77843-4458
phone: (979) 845-3177
fax:  (979) 458-3499

>>> "Hess, Mary" <> 09/25/00 02:24PM >>>
I've received some responses asking why we used xylene for Masson's
trichrome instead of pro-par in the first place. Initially it was because I
got the Masson's Trichrome protocol and the kit from another person at my
company who was very much into using only 'pure' reagents (ie, expensive
absolute alcohols, xylene, etc) to ensure the quality of his slides. And the
quality is very good when you use those solutions. When I started, I quickly
switched to our normal alcohol and found no difference between his slides
and mine. I have now tried to do our Masson's trichrome using pro-par and
refrax mount instead fo xylene and permount. My initial results aren't too
Recently we have found eosin 'bleeding' from our H&E tissues when the slides
were mounted (for which we used Pro-Par and Refrax mounting media). I
switched out everything (from water, to alcohol, to Pro-Par, to eosin and
hematoxylin) in our H&E stations and still had the problem. When I did the
trichrome with the Pro-par,  the Biebrich Scarlet Acid Fuscin bled into the
mounting media. I don't know what it is. If you guys do I would be grateful.
And in the end, it was the guy who started me on the stain who kept me using
xylene. I don't want to keep using it, but so far, it seems like I'm going
to have to. I hope this 'bleeding' is something I'm doing wrong and is
easily fixable, I'd rather it be my fault. Thanks for the help so far....
-Mary Hess, San Diego, Ca.

<< Previous Message | Next Message >>