Re: Lillies fixative
|From:||"J. A. Kiernan" <email@example.com>|
On Thu, 28 Sep 2000, Jennifer Englin wrote:
> I read you response to the fatty tissue. What is the recipe
> for Lillies fixative ...
Lillie described several fixatives. The one most widely
used is a phosphate-buffered formaldehyde solution in which
the net osmotic pressure of solutes other than HCHO is
slightly greater than that of mammalian extracellular fluid.
Abbreviations like NBF are often used for such liquids.
My humble opinion is that Lillie's best all-round fixative
was his formalin-calcium acetate, which combines being
extremely easy to make (formalin, calcium acetate and water)
with improved preservation of phospholipids (because of the
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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