Re: Immunohistology

From:"Circle :>" <>

Hi all,

I am new to IHC, too. I would like to ask if anyone of you have a good
protocol for immunohistochemistry to stain the rat brain tissue and rat 
aorta. I felt confusing with my friends. Some of them suggest that I
should add OCT compound while the other said no need. At the same time,
some of them suggested that I should add something more.

I felt very confusing.

Anyway, my rat's tissue has been frozen in liquid nitrogen and now stored
in -70^C. What should I do next? I would like to stain an Enzyme.

Thank you!

With reference to Anila's question, one of my friend who stained the liver
tissue used 4 micro meter. 


On Mon, 25 Sep 2000, Anila Syed wrote:

> Hi all,
> I am relatively new to immunohistology, but need to stain mouse liver for cysteine dioxygenase.
> I would like to ask a few very basic questions please.
> If anyone has experience of using these techniques, I would be grateful of their assitance.
> First of all, what would be a good section thickness?
> Secondly, is it best to use coated slides or not and if so, what is the best coating agent?
> Many thanks in advance for your help,
> Anila Syed

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