RE: grids for cell counting
|From:||RUSS ALLISON <Allison@Cardiff.ac.uk>|
Another good one John!
Now I'll add yet another!
when trying (pre-digital age) to measure the length of basement
membrane, I used to make transparent image of section as you
suggest (actually, I used to project the image up between my legs
onto a glass screen and trace it).
Then I used a cotton reel attached to a darning needle with the
thread comming thru' the eye and sticking to the adhesive covered
image as a I traced along the BM.
When completed, it was easy to strip the thread from the image,
straighten and measure against a ruler.
Simple maths then allowed a count of nos. of cells per mm of BM!
Thank goodness for digital imaging, etc.
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