We had this problem a couple of years ago and were able to attribute it to
all of the ROH not being removed from the sections.  Once we increased our
time in the first and second xylenes, the problem was solved.


Cheryl George, BS, HT (ASCP)
Histology/Cytology Supervisor
NHML
Manchester, NH
cgerorge@optima.org
603-663-2686


> ----------
> From: 	Jill Songer[SMTP:jtsonger@vt.edu]
> Sent: 	Wednesday, September 27, 2000 8:31 AM
> To: 	Wylie, Teresa P; 'histonet@pathology.swmed.edu'
> Subject: 	Re: eosin bleeding
> 
> We also occasionally experience this problem and would be interested in
> the 
> responses.
> 
> 
> 
> At 01:46 PM 9/26/2000 -0400, Wylie, Teresa P wrote:
> >We have also had a problem with Eosin bleeding on routine H&E slides.  We
> >use Xylene on the stainer and Mounting Medium from Richard-Allan on an
> >automated coverslipper.  We have three absolute alcohol stations after
> the
> >Eosin and have tried using fresh alcohols and xylenes for each staining
> >rack, but still have the problem.  Sometimes it is more noticeable than
> >others.  Could it be the mounting media?  I would appreciate any
> >suggestions.
> >
> >Thanks,
> >Teresa
> >
> >
> >Teresa P. Wylie
> >Scientist
> >Medicine Safety Evaluation
> >GlaxoWellcome
> >RTP, NC
> >
> >
> >
> 
> 
> **************************************************************************
> Jill Songer HT (ASCP)
> Anatomic Pathology Lab Supervisor
> Veterinary Teaching Hospital
> Virginia Tech
> Blacksburg, Virginia 24061
> 
>