We had the "bleeding" problem when we used the mounting medium from Richard
Allen.  We replaced it with Permount and have not had any further problems.

Jennifer MacDonald

> ----------
> From: 	Wylie, Teresa P[SMTP:tpw12762@glaxowellcome.com]
> Sent: 	Tuesday, September 26, 2000 12:46 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	eosin bleeding
> 
> We have also had a problem with Eosin bleeding on routine H&E slides.  We
> use Xylene on the stainer and Mounting Medium from Richard-Allan on an
> automated coverslipper.  We have three absolute alcohol stations after the
> Eosin and have tried using fresh alcohols and xylenes for each staining
> rack, but still have the problem.  Sometimes it is more noticeable than
> others.  Could it be the mounting media?  I would appreciate any
> suggestions.
> 
> Thanks,
> Teresa
> 
>  
> Teresa P. Wylie
> Scientist
> Medicine Safety Evaluation
> GlaxoWellcome
> RTP, NC
>  
>  
> 
> 
>