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<DIV><FONT color=#0000ff face=Arial size=2><SPAN
class=288364210-27092000>Shelley:</SPAN></FONT></DIV>
<DIV><FONT color=#0000ff face=Arial size=2><SPAN
class=288364210-27092000></SPAN></FONT> </DIV>
<DIV><FONT color=#0000ff face=Arial size=2><SPAN class=288364210-27092000>We do
the Dahl's method for calcium which uses Alizarin Red S. It is a fast,
simple and beautiful stain. Our reference is from the AFIP Manual, 3rd
edition, 1968. Let me know if you need me to send you the
procedure.</SPAN></FONT></DIV>
<DIV><FONT color=#0000ff face=Arial size=2><SPAN
class=288364210-27092000></SPAN></FONT> </DIV>
<DIV><FONT color=#0000ff face=Arial size=2><SPAN class=288364210-27092000>
<P><FONT size=2>Jeanine Bartlett<BR>Centers for Disease Control<BR>Atlanta,
GA<BR>jbartlett@cdc.gov </FONT></P> </SPAN></FONT></DIV>
<BLOCKQUOTE>
<DIV align=left class=OutlookMessageHeader dir=ltr><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> Shelley Sheridan
[mailto:sksherid@yahoo.com]<BR><B>Sent:</B> Tuesday, September 26, 2000 4:16
PM<BR><B>To:</B> HistoNet Server<BR><B>Subject:</B> Alzarin Red stain for
Calcium<BR><BR></DIV></FONT>
<P>Does anyone know about the Alzarin Red stain for Calcium? One of our
post-docs is trying to stain Calcium in paraffin embedded human brain
tissue. We have no experience with this stain and are unable to assist
him. Any information would be appreciated. Thank you.</P>
<P> </P><BR><BR>
<P>Shelley K. Sheridan<BR>Research Specialist<BR>Center for Neurodegenerative
Disease Research<BR>University of Pennsylvania Medical School<BR>Philadelphia,
PA 19104<BR>Phone:(215)614-0051 Fax: (215) 349-5909</P>
<P><BR>
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