Light Sensitivity during IHC

From:Nima Farsinejad <>

I have just run accross a protocol which mentions applying DAB Substrate
in the Dark due to light sensititvity. The protocol follows the
ABC/peroxidase method. I am a little confused about this footnote and I
was wondering if anyone could clarify this to me. Sincerely, 

Nima Farsinejad
Research Specialist
Digestive Diseases
Emory University
Atlanta, Georgia

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