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Hello out there!!

I am looking for a good protocol or technique for antibody labeling of
mouse coronal x-sections starting from frozen(cryostat) sections.  The
protocol I am using now does not use a delipidization step and
inclubates the sections with antibody in a solution of
TBST(1%) milk(2%) and 1% DMSO.  Primary and secondary antibody
concentrations are in the high end range( 1:250)  which doesn't seem to
make a difference.  Any suggestions or protocols would be greatly
appreciated.  I am currently just using general nerve tract antibodies
such as NF and tubulin(acetylated).


thanks in advance


sam thompson Ph. D
university of utah
dept biology

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