Frozen IHC

From:Jim Manavis <>

Can someone tell me their experience with frozen sections which are post
fixed in cold acetone prior to applying immuno histochemistry.
Which procedure do you find is superior?  Does it also vary depending on the
Ab used?
Post fix immediately, dry, leave them say, overnight in the -20 freezer, and
then apply IHC?
Do you air dry after sectioning, store overnight, then post fix just prior
to IHC?
Do you section, post fix and then apply IHC on the same day?

Thanks, Kathy Cash

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