Calcium staining in brain sections

From:=?iso-8859-1?q?Dr=20Murray?= <>


I am looking for calcium staining that can pick up
calcium in the brain. If anyone has experinence in
this are could you please send me protocols

I have found several methods in Thompson 1966
"Selected histochem and histopath methods". However I
am not sure if they have been used in the brain, to
see low quantities of calcium in the brain regions.

These methods include:
Von Kossa (modified Mallory)
Alizarin Red (McGee Russell)
Murexin (Kaufman and Adams)
Napthochrome Green B stain (Denz, modified by Pearse)

I much appreciate any help or advice in this matter,


Ian Murray

Dr I.V.J Murray, Postdoctoral Fellow
Centre for Neurodegenerative Disease Research,
3rd Floor Maloney, University of Pennsylvania 
36th and Spruce St, Philadelphia, PA 
19104 USA
tel (215) 662-3292                 fax (215) 349-5909 

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