Freida Carson's book gave an excellent recipe for alizarin red for calcium.
 There are some considerations on how the reagent is made up, pH is critical.

You could also do a Von Kossa's for calcium, which, in my opinion, easier
to do, giving black deposits that can be counterstained with H&E.


Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303