Plastics interfer with immunostaining by preventing immunoglobulins access
to antigenic sites.  Plastic should be removed, PMMA can be dissolved away,
GMA cannot, other plastics go through stringent etching methods potentially
hard on antigenic sites.

Neil Hand J of Histotechnology, a few years back, does PMMA technic with
antigen retrieval on a huge panel of antibodies very successfully.


Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303