intern seeks help...
|From:||Heather Hall <firstname.lastname@example.org> (by way of histonet)|
My name is Heather Hall and I am presently doing an
internship at Primedica Inc., located in Worcester,
Mass. One of the required slides is skin stained with
trichrome. For some reason, I cannot get this slide
to come out right. I first tried Masson's procedure,
with kit reagents. The slides appeared as though they
had not been differentiated in
phosphotungstic/phosphomolybdic acid long enough, as
the collagen bundles were retaining the scarlet-acid
fuchsin in their centers. My first step to correct
the problem was to increase the time in the p/p acid;
that didn't work. Next I made up fresh reagents;
didn't work. Altering the times in the other reagents
didn't work either, so I tried the Gomori's procedure.
When the Gomori's did not work, I changed species
(first species used was pig, second was human). The
same problem was occuring in the human tissue also.
Since the control slides appeared to coming out fine I
tried a third species. I tried rat skin hoping that
it might have a thinner connective tissue layer and
permeability would be altered, but that came out as
badly as the others. I don't know what to do next to
try and fix this problem. Any suggestions would be
very much appreciated. Thanks for any help you may be
able to give.
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